FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7393860
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18450.013.4522531
AAACGGG20550.013.1337447
AACGGGA17400.012.9261718
GAAACGG20900.012.7011486
ACGGGAT12650.012.2681119
CGGGATT11850.011.76772510
GGAAACG23050.011.2505125
CGGGAAT7550.09.83073810
ACGCCCG4355.7298166E-109.82541834
CGCGCCC5900.09.53166633
CGGCTAC2851.7642797E-59.47146633
ACCGCGC6500.09.34396631
CCCGTTA2200.00144980989.2303571
CACCGCG7150.09.12373730
CGCCCGG9950.08.81714935
AACGGGT4355.896254E-88.7897978
GCGTGAG19350.08.71764922
CCCGATT2851.7650439E-48.7085641
ACAGGCG28000.08.51465318
CGTGAGC20350.08.51031123
Produced by FastQC (version 0.11.5)