FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815571
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATC2203.5761332E-914.31916438
CGTCCCG1758.180614E-714.14362135
GAAACGG20100.013.3207356
CGTTGGA19850.013.2904751
AAACGGG20700.012.823587
AACGGGA16900.012.6454578
ACGGGAT12300.012.6194849
CGGGATA2156.0233833E-712.55568510
GGAAACG21400.011.8822675
TCCCGAT2758.091229E-811.45533237
CGAAACG1450.006824301610.8633725
CGGGATT12050.010.8277610
CCCGTAA2709.030553E-610.0214991
GTCCCGA2504.35242E-59.90053536
CGGGAAT6950.09.71033210
ACAGGCG20700.09.56334818
CCGGCTA6900.09.457170538
CCCGGAC2150.00118231169.4388531
ACCGCGC4551.2896635E-99.39521531
CCGATCT2658.2347375E-59.34053839
Produced by FastQC (version 0.11.5)