FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134182
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21250.012.8482361
AACGGGA17600.012.5241298
AAACGGG20800.012.4356537
GAAACGG21550.012.3168796
ACGGGAT13400.011.7497169
GGAAACG23600.010.89860255
CGGGATT12850.010.851171510
CCCGGAT2708.955718E-610.0284661
ACGGGTA2109.752506E-49.6395639
AACGGGT4253.9981387E-99.5261558
CGGGAAT7800.09.5149710
CCGGCTA5953.6379788E-129.0717938
ACGCCCG3053.933667E-58.84871334
CCGGTTT2300.00216969348.830353
CGGGATA3104.762384E-58.70577910
CCCATAA18750.08.6645951
GCGTGAG13450.08.52805822
ATGCGCC2400.00328922238.43399924
CGGGTAT2400.00329019188.43372310
CGTGAGC14400.08.27781323
Produced by FastQC (version 0.11.5)