FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9238098
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG26600.012.5175087
AACGGGA22600.012.2440498
ACGGGAT17500.012.0842089
CGTTGGA29550.011.9930051
GAAACGG28400.011.3280596
GGAAACG29100.011.2101855
CGGGATT16750.010.87924110
CGGCTAA11200.010.24453639
CCGGCTA11050.09.97640238
CGGGAAT9900.09.77150910
CCCGTAA4509.949872E-109.5307461
CTCGTAT2606.700738E-59.51845644
CACCGCG7100.09.18904630
CCCGAAC3258.095438E-69.0291281
CGCCCGG10550.08.74322335
AAGCGGG6950.08.7401087
CCCTAAT19100.08.6273091
AACGGGT5302.220986E-98.4895098
ACTGCGC9050.08.45203131
GCCACCG19750.08.4293328
Produced by FastQC (version 0.11.5)