FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440069
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4050.018.87708944
TCTCGTA4450.017.68557443
ACGGGAT13100.016.8214159
AACGGGA16300.015.5882938
GATCGGA5550.015.0765461
TCGTATG5250.015.03533845
CGGGATT12300.014.99051210
CGTTGGA19600.014.8842431
CGGGATA2304.292815E-1014.66463110
AAACGGG21050.014.4208017
GAAACGG22050.013.3621076
TCGGAAG6450.012.9564533
GGAAACG22500.012.8490665
AGTCACG6350.012.74813528
ATCGGAA6600.012.6619882
ATCTCGT6050.012.63712142
GTCACGT6050.012.26525129
ACACGTC6550.012.0153913
AACGATC6600.011.58501638
AAACGAT6850.011.49050637
Produced by FastQC (version 0.11.5)