FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11915315
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA32550.013.5616471
ACGGGAT21350.013.2782439
AACGGGA30650.012.2589688
CGGGATT20000.012.14964410
AAACGGG36300.011.7763657
GAAACGG37450.011.3545226
GGAAACG37900.011.0416145
CGGGATA4551.2369128E-109.88981910
ACCGCGC7750.09.87042931
CACCGCG8600.09.41809130
GCCACCG25150.08.76691728
TCTCGTA5555.857146E-108.51319143
CCCGGAT4551.19198376E-78.4148211
GATTTCG14300.08.3387065
CCGCGCC8950.08.29563632
GCGTGAG28150.08.15230822
CCCGAAA5057.406379E-88.0276511
CCGGCTA10150.07.9798638
CGTGAGC29250.07.922644623
ACAGGCG37200.07.862474418
Produced by FastQC (version 0.11.5)