FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4926440
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT10250.014.7029139
CGTTGGA15900.013.9010171
ACGCTAG1301.9330578E-413.84200727
TATGCCG2306.510163E-913.7220513
AACGGGA14100.013.7195728
CGGGATT9800.013.54186110
ACTGCGC4650.013.06060231
AAACGGG17450.012.6325747
GAAACGG17950.012.5314466
CGTATGC2552.7415808E-812.382421
CACTGCG5600.011.64829630
CGTGAGC14950.011.58515723
CCCGATT1953.7569087E-511.5659961
GCGTGAG14250.011.52286322
CGCCCGG6100.011.06248435
GGAAACG19450.011.0057515
ACCGCGC4304.1836756E-1110.46198131
CACCGCG4501.0004442E-109.99700530
CCCGAAT5301.8189894E-129.7874521
CTGCGCC5301.8189894E-129.76122732
Produced by FastQC (version 0.11.5)