FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9176734
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.024.10638245
GATCGGA5600.022.9250451
CGGCTAC7200.017.81145333
CTCGTAT7400.017.33053442
ACGGCTA7450.017.21375332
CGTATGC7850.016.33706344
GTCACGG8300.015.1798329
TCTCGTA8500.015.08734741
ATCTCGT9100.014.09257740
TCACGGC9000.013.99917630
CGTTGGA25650.013.8737261
TCGTATG9250.013.86442643
ATCGGAA9600.013.842172
TCGGAAG9300.013.7904883
ACGGGAT15000.013.6496439
CGTCTGA9650.013.52266816
AGTCACG9350.013.23451428
AAACGGG26400.012.9541367
AACGGGA20850.012.733418
CACGGCT10300.012.66915731
Produced by FastQC (version 0.11.5)