FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6243707
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18750.014.6640871
AACGGGA15750.014.2841228
AAACGGG18750.013.3185167
GGAAACG20150.012.6213115
GAAACGG20250.012.5585816
ACGGGAT11300.012.5429529
CCCGCGC1350.00396152911.6858631
CGGGAAT7750.011.61170310
ACGGGAA7100.011.4072719
CGGGATT12150.011.29512810
ACCGCGC4850.010.66899931
CACCGCG5800.010.47397830
GCGTGAG17600.010.22646122
GCCACCG16550.010.06026928
CGTGAGC18150.010.04052523
ACAGGCG21700.09.95233118
CGCCCGG6250.09.71938435
GATTTCG7300.09.5574045
ACGCCCG3603.21872E-79.37425634
CGAGAAT4152.6588168E-89.2320331
Produced by FastQC (version 0.11.5)