FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11378969
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA32450.012.7660351
AACGGGA27050.011.8944128
GAAACGG34050.011.4313986
AAACGGG34050.011.2992437
GGAAACG34550.011.1393465
CCCCGAT4253.6379788E-1211.1245711
CGCGCCC6500.010.73018633
CGGGAAT12800.010.72240410
GCGTGAG27200.010.67037722
ACGGGAT20850.010.3595149
ACAGGCG37700.010.32450918
CGTGAGC28650.010.05176623
CCGCGCC8600.09.941277532
GCCACCG23350.09.6354528
ACCGCGC8450.09.58523731
CACCGCG9000.09.24949830
CAGGCGT35050.09.24355719
CGGGATT19650.09.045588510
AGGCGTG36500.08.99959120
CCCCGAA4355.8034857E-88.798581
Produced by FastQC (version 0.11.5)