FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533535
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA13650.013.2157181
AAACGGG15200.011.3941597
AACGGGA12150.011.2924648
CCCCGAT1802.299804E-411.274661
CGATGTA2251.3649707E-510.99760334
CCACGAA3154.43124E-810.7377711
CGGGAAT5700.010.65427910
GGAAACG15900.010.3493995
ACGGGAT8700.010.0827869
GAAACGG16300.09.9354986
ATCTCGA2555.428899E-59.70291130
CGTATGC2109.7423885E-49.64054344
ACCGATG1900.00463367079.47099332
CTCGACA1900.00463367079.47099332
CGAAGGT1950.0056234019.2510031
CTCGTAT2000.00706550768.99754242
TCTCGAC2000.00706678688.99734431
CGGGATT8500.08.99694710
CCCGAAA2050.0084779088.7997341
CACCGAT2050.0086521658.77789631
Produced by FastQC (version 0.11.5)