FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7387251
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23100.014.5420571
ACGGGAT15900.014.1468519
CGGGATT14650.013.81852710
AACGGGA21350.013.8016278
AAACGGG25400.013.5495967
GAAACGG26200.012.966796
ACGCCCG3850.012.27823534
GGAAACG27250.012.2196265
CGTGAGC19100.011.42556223
CCCGAAA3753.6379788E-1111.4228341
GCGTGAG18100.011.3091922
CGCCCGG7750.011.03743535
ACCGCGC5600.010.84581131
CACCGCG6100.010.69493130
CCCCGAA3501.8839273E-810.3063161
CCCCCGT3755.0422386E-910.2204311
CCGGCTA7200.010.00428538
ACAGGCG26450.09.95013118
GCCACCG16650.09.86488228
GAGCCAC54150.09.63990726
Produced by FastQC (version 0.11.5)