FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6642878
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21600.013.923111
AACGGGA19050.013.4556928
AAACGGG22700.013.2735827
ACGGGAT13450.013.2069029
GAAACGG23200.012.6031486
CGGGATT12750.012.16844510
GGAAACG25000.011.5679595
ACGCCCG4651.8189894E-1210.63918434
CGGGAAT9150.010.566810
CGCCCGG7850.010.02621635
CCCCGTA2057.4478344E-49.9272051
GCCACCG15200.09.91197128
CGCGCCC3901.0002623E-89.80218633
ACCGCGC5750.09.77630731
GCGTGAG17200.09.02089222
CGGCTAA7500.08.99481139
ACGGGAA9500.08.9940679
CACCGCG6550.08.92554430
CGTGAGC18200.08.772346523
CCGGCTA7750.08.70472238
Produced by FastQC (version 0.11.5)