FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5933950
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7150.032.087542
TATCTCG7750.030.18401539
CGTATGC7700.029.79553444
TCTCGTA7700.029.2113141
TCGTATG8550.026.83340543
GATCGGA9850.024.503281
ATCTCGT9400.023.92841140
ATCGGAA10400.022.9728782
ACACGTC10550.022.38610613
TCGGAAG10700.021.9074823
GTATGCC11400.020.75684545
CGTCTGA12250.019.46307616
CGGAAGA12600.018.7828564
ACGTCTG13050.018.44229715
CACGTCT13350.017.85937914
GCACACG14750.016.31674411
CACACGT15700.015.61595612
CACTAGC15450.015.14072131
GCTTATC16100.015.08840336
ACTAGCT15950.014.94813232
Produced by FastQC (version 0.11.5)