FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433651
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA6000.012.3952671
CGTTGGA38650.012.07018851
ACGGGAT24650.012.0491869
AACGGGA32750.011.7485848
AAACGGG38750.011.6124827
CGGGATT24600.010.88460210
ACGCCCG8250.010.63577934
GAAACGG42650.010.4978616
GGAAACG44950.09.7609265
CGTATGC6100.09.58920945
CACCGCG9400.09.09521130
ACCACGC27750.08.99930531
CCGGCTA15100.08.94017238
GCGTGAG30050.08.90926322
ACCGCGC9150.08.85177531
CGGCTAA15000.08.84977839
CGCCCGG14450.08.71930935
ACAGGCG44500.08.64520618
CGTGAGC32050.08.35333323
CCCTAAT30000.08.0381431
Produced by FastQC (version 0.11.5)