FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867147
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24600.014.4719161
AAACGGG23900.014.4036747
AACGGGT5150.013.5426578
GAAACGG25150.013.5147876
ACGGGAT15500.013.4984559
AACGGGA20200.013.4766958
GGAAACG27150.012.6022135
CGGGATT13900.012.13889910
CACCGCG5800.012.02448630
ACGCCCG3950.011.96065634
ACCGCGC5850.011.92171331
CGCGCCC4750.011.36712133
CGCCCGG7300.010.78643735
ACGGGTT5550.010.5393359
GCGTGAG20050.010.43581222
CGTGAGC21400.010.09236723
CGGGAAT9250.09.97183110
CCGGCTA7700.09.93405438
CCGCGCC6800.09.92533532
ACAGGCG26650.09.87760918
Produced by FastQC (version 0.11.5)