FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257194
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20750.012.9620211
AAACGGG20400.012.1253837
AACGGGA17300.011.828388
GAAACGG21850.011.7342456
ACGGGAT13050.011.7173229
CGGGATT11800.011.62459510
GGAAACG24200.010.7300675
CCCCGAA3401.3109093E-79.9714011
CGTAGTA2606.371443E-59.5623181
AACGGGT4458.8439265E-99.0958338
CGCGCCC4304.9007213E-88.8907933
GCGTGAG16200.08.74527522
CCGCGCC5952.6375346E-108.3147232
CACCGCG5952.6375346E-108.3147230
CCCTAGT12850.08.0909321
ACAGGCG25000.08.00539918
ACCGCGC5901.9590516E-98.0040431
ACTGCGC5657.396011E-97.96019131
CGTGAGC16450.07.92890123
ACCGTGC6002.7175702E-97.87063931
Produced by FastQC (version 0.11.5)