FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394660
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6200.023.24355945
CTCGTAT7300.021.26179343
TCGTATG7800.018.74606144
TCTCGTA7900.018.2232842
ATCTCGT8700.016.54746241
CGTTGGA22850.013.8214491
AATCTCG12000.012.55926740
AAACGGG24950.011.9906577
GAAACGG25850.011.9212546
AACGGGA20150.011.6095228
GGAAACG26050.011.4214635
ACACGTC14350.011.12916212
ACGGGAT16150.011.0029889
CGTCTGA14500.010.85883215
CGGGATT14100.010.3693210
AACGGGT6300.010.3541278
ACGTCTG15500.010.3035214
ATCGGAA14150.09.8843251
TCGGAAG15050.09.5869062
CACGTCT16850.09.47795113
Produced by FastQC (version 0.11.5)