FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6637879
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT12700.012.3997099
AAACGGG20450.012.3208847
AACGGGA17250.011.9981958
GAAACGG22350.011.8784816
CGTTGGA20800.011.4919751
CGGGATT12600.011.24830710
GGAAACG24250.010.4839855
AACGGGT4501.0568328E-99.4985718
CGAAGAA5251.6128979E-88.1610571
GCGTGAG16150.07.94010122
ACAGGCG22800.07.89372718
CGTGAGC16250.07.891179623
CGAGGAT3451.6069938E-47.843581
ACGCCCG4051.5688152E-57.77895134
CGCCCGG7354.0017767E-117.653004635
CGGGAAT7151.8007995E-107.55131110
CGGGATA2700.0094499597.498871310
CCCGAAT6203.918649E-87.27428771
GAGCCAC48350.07.07267826
CACCGCG4151.7822838E-47.04867230
Produced by FastQC (version 0.11.5)