FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6059950
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16850.014.6281861
AACGGGA15500.013.3468918
AAACGGG17850.012.9754667
GAAACGG18400.012.5914576
ACGGGAT10900.012.58424959
CGGGATT10750.012.1323110
GGAAACG18950.012.0292145
CGGGAAT6600.011.24330410
CACTGCG5403.6379788E-129.57984330
CGCTAAA2150.00115160099.4660321
GCGTGAG12150.09.43936922
ACTGCGC4355.9644663E-99.30710731
ACGGGAA7550.09.2329139
CCCGGAT2453.682767E-49.2299171
CGTGAGC13200.09.19959823
CCCCGAA2504.507666E-49.0453181
CCCATAA19650.08.9762711
ACAGGCG16800.08.96787518
CCTCGAG2350.002587988.6604111
CCCTATT15100.08.5361451
Produced by FastQC (version 0.11.5)