FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8334969
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC2912083.4938102349270883TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA310000.044.239811
ATCGGAA311850.043.979792
TCGGAAG312950.043.8971063
ACACGTC311000.043.6580113
CGGAAGA314900.043.6467134
CGTCTGA310050.043.6261916
CTCGTAT308350.043.5206442
TCTCGTA308550.043.50779741
ACGTCTG311850.043.4248815
CGTATGC309550.043.3968444
TCGTATG309850.043.3825643
TCACGAT309300.043.32332635
CACGTCT313500.043.23938814
ATCTCGT310500.043.19833440
GCACACG316100.043.1529111
GTATGCC311950.043.04879445
CATCACG311750.043.0349233
ACGATCT311950.042.93984237
CACACGT317600.042.92785312
CACGATC312050.042.8689236