FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022569
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4200.017.67168442
TCTCGTA4350.016.02823641
TCGTATG5500.014.31260443
CGGGATT12600.013.92304710
ACGGGAT14600.013.8643619
CCCCGAT2358.694769E-913.4422941
AACGGGA19450.013.4137028
CGTATGC5550.013.37316644
AAACGGG23450.013.3315967
CGTTGGA21600.013.2667081
GATCGGA5500.013.1280581
GAAACGG25050.012.2135066
ACACGTC6750.011.66203913
ATCTCGT6250.011.515758540
GGAAACG25100.011.5009975
ACCGCGC6150.010.9731331
CATCTCG7250.010.54783839
ATCGGAA6800.010.2813942
CACCGCG6650.010.14808530
CCCGGAT2901.9057352E-610.114831
Produced by FastQC (version 0.11.5)