FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6679610
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4600.015.65047444
TCTCGTA4800.015.46695343
TCGTATG5200.014.27793545
CGTTGGA18750.014.0787331
ACCGCGC4950.012.72674831
CACCGCG5350.012.616302530
ACGGGAT12250.011.9376579
AACGGGA16700.011.5857418
AAACGGG20450.011.4414727
CGGGATT12550.011.11449610
ATCTCGT6500.011.07572142
ACGCCCG3052.8516297E-811.0646234
GCGTGAG17000.010.98442922
GAAACGG21650.010.8085166
GCCACCG13800.010.76026528
CGCCCGG6550.010.64817635
GGAAACG21300.010.4579425
CGTGAGC17800.010.36435323
GCGCCCG4304.620233E-109.94100434
CGCGCCC3653.727473E-89.86260733
Produced by FastQC (version 0.11.5)