FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5752213
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA17850.014.6677551
CGGGATT10900.013.8238910
ACGGGAT11750.013.5894689
CGTTTCG1250.002209796312.6328374
AACGGGA17450.012.630278
AAACGGG20450.012.0971017
ACCGCGC4051.8189894E-1211.66401931
CCCGTAA2553.6210986E-711.5066011
CACCGCG4450.011.1210730
GAAACGG22450.010.9217966
GGAAACG22200.010.771245
CCGCGCC4701.8189894E-1210.52769432
CCCGATT2301.9898517E-49.8133221
GCCACCG12550.09.67865628
GATTTCG7300.09.5796955
CCTCGAT1900.00450515379.50342751
GCGTGAG14650.08.90381422
CGCGCCC3807.067192E-78.87801833
ACAGGCG19100.08.83118
CCCGTGT4406.7000656E-88.7204751
Produced by FastQC (version 0.11.5)