FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7300657
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG2005.2750693E-1116.86690935
CGTTGGA18600.013.9816181
AACGGGA17000.013.2273188
ACGGGAT13100.012.3589599
GAAACGG21500.012.3440996
AAACGGG21400.012.0838357
CCCGATC3001.7553248E-911.99408238
GGAAACG22600.011.4910875
CCGATCT3501.6843842E-910.92310739
CGGGATT12700.010.62351410
GTCCCGA3002.711513E-710.494822536
TCTCGTA3257.304698E-810.37835643
TCGTATG3401.3544377E-79.94910645
CCGGCTA7200.09.68272338
CTCGTAT3301.0016174E-69.53976744
CCGTCCC3951.2680903E-79.10955434
CGGGAAT7950.09.05114610
GATCGGA4102.0627522E-78.8248771
TCCCGAT3603.2579446E-68.74568537
CGGCTAA7450.08.452147539
Produced by FastQC (version 0.11.5)