FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8508214
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22900.014.4701591
ACGGGAT15600.012.8327339
AAACGGG24750.012.4513667
AACGGGA20200.012.3601968
CGGGATT14100.012.28359210
GAAACGG26850.011.9000416
GGAAACG27400.010.7579045
CGGGAAT7550.09.83152110
CGCCAAT2555.3028343E-59.7239771
GCGTGAG21400.09.67048522
CCCCGAA3806.7435394E-89.4913461
ACCGCGC4601.5752448E-99.29159631
CCACGGT4651.8444553E-99.2106881
CGTGAGC21950.09.01968223
TATGCCG2751.2337722E-49.00050453
GTTCGAG13100.08.92875514
GCCACCG18150.08.92575428
CCCCGAT2801.4715517E-48.8557651
CCCAAAG98600.08.6189791
CGGGATA4203.2340176E-78.56888710
Produced by FastQC (version 0.11.5)