FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8451515
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG4200.025.1774735
GTCCCGA5050.021.385236
TCCCGAT5400.019.99912337
CTCGTAT5350.019.76549144
CCGATCT5250.019.71342339
CCCGATC5450.018.98999438
TCTCGTA6100.018.44181643
ACCCGTC6150.017.92604432
ATCTCGT6550.017.86181342
TCGTATG6300.017.14210745
GATCGGA6400.015.4847451
CACCCGT7000.015.10648231
CCCGTCC7600.014.80198433
CCGTCCC8200.013.71891234
TCACCCG7550.013.41000930
ATCGGAA8400.013.1268332
ACGGGAT15100.012.6650099
TCGGAAG8550.012.6333443
AAACGGG25050.012.57437
CGTCTGA8950.012.56928216
Produced by FastQC (version 0.11.5)