FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10935219
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1750861.6011201970440647TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG192300.043.4011845
GATCGGA196600.043.300271
TATCTCG193350.043.17689541
CTCGTAT193700.043.0894344
TCTCGTA193700.043.07722543
TCGGAAG197850.043.014133
ATCGGAA198550.042.908662
ATCTCGT195450.042.70147342
ACACGTC199650.042.6128913
CGTCTGA199450.042.4864416
CGGAAGA200450.042.442244
ACGTCTG200300.042.3173715
CACGTCT201800.042.10314614
GCACACG206000.041.37577411
CACACGT207750.041.04889712
AGAGCAC221100.038.9068268
AGCACAC222100.038.5486310
GAGCACA224850.038.1478969
TCTGAAC223250.037.9470118
GTCACAT223150.037.83331329
Produced by FastQC (version 0.11.5)