FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4852763
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG106800.22008080757292287TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14400.037.95588344
TCTCGTA14850.037.10863543
TCGTATG15050.036.39609545
GATCGGA15800.036.2595021
ACACGTC16650.034.04146213
ATCGGAA17050.033.733472
ATCTCGT16350.033.70451742
TCGGAAG17200.033.570423
AATCTCG17050.032.58493441
ACGTCTG17600.032.45958315
CGTCTGA17400.032.44489316
CGGAAGA18500.030.8456554
CACGTCT18700.030.67047514
GCACACG19300.029.6004511
CACACGT20400.028.22485412
AGTCAAC21750.026.06013334
GTCAACA25450.022.18327935
AGCACAC27600.021.4322910
CAGTCAA26600.021.30856533
AGAGCAC27800.021.0353838
Produced by FastQC (version 0.11.5)