FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10036363
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13400.031.39916642
TCTCGTA13450.031.28197541
CGTATGC14150.030.05247344
TCGTATG14550.029.69034243
ATCTCGT15900.026.88631640
GATCGGA16150.025.3691121
ATCGGAA17300.023.9341452
ACACGTC18250.023.67069613
CGTCTGA18750.022.79948216
TCGGAAG18200.022.750593
AATCTCG19300.022.14975739
CGGAAGA20150.020.548924
ACGTCTG21250.020.54070915
GTATGCC21050.020.20154245
CACGTCT22250.019.31416914
GCACACG23500.018.86099211
CACACGT24500.018.09115612
GTCACAC33100.013.25472229
GAATCTC37450.012.01580938
AGAGCAC39500.011.4488798
Produced by FastQC (version 0.11.5)