FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14219577
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT23300.012.8705939
CGTCGAC850.002427661812.4758111
AACGGGA31750.012.2315238
CGGGATT20000.012.07036310
CGTTGGA38400.011.9931161
AAACGGG39750.011.8067867
CGGGATA5950.011.08817810
GCGTGAG20950.011.02322122
GGAAACG39500.010.9333175
GAAACGG42000.010.817736
CACCGCG7400.010.73934530
ACAGGCG28150.010.33354518
CGTGAGC23400.010.31767523
GCCACCG19600.010.09855328
CCCAAAG107800.09.4296041
GAGCCAC67250.09.32039626
CCACCGC15350.09.28014929
AGGCGTG28850.08.7843520
CAGGCGT27400.08.70180319
ATAGGCG3654.5110937E-108.62685318
Produced by FastQC (version 0.11.5)