FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14446177
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5700.016.1193541
GTCACCG7900.012.14995229
CGGGATT21650.011.91515710
ACGGGAT22250.011.8634749
CGTTGGA33650.011.2352131
AACGGGA29950.010.6161428
AAACGGG36000.010.5817857
ACACGTC9650.010.25742413
ATCGGAA9150.010.16251852
CACCGCG9100.010.05326630
GAAACGG37900.09.9325846
GGAAACG37850.09.9058755
TCGGAAG10200.09.704533
CGTCTGA10350.09.41910716
ACAGGCG37000.09.36368918
GCGTGAG26400.09.31678622
ACGTCTG10900.08.94386215
CGTGAGC27350.08.88355723
AAGCGGC10300.08.8822573
GCCACCG23750.08.8406928
Produced by FastQC (version 0.11.5)