FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7901571
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5150.015.28398541
TCGTATG5900.014.4846343
CTCGTAT5550.014.18243642
CGTTGGA20450.013.3699131
ACGGGAT14150.013.1902639
CGTATGC6150.013.16346544
AACGGGA18850.013.1223968
CGGGATT13600.012.89680110
AAACGGG21800.012.6876287
GAAACGG23100.012.2665766
ATCTCGT7300.011.39868240
GGAAACG24650.010.9941245
GATCGGA7900.010.8690861
TATGCCG3205.549009E-810.57484945
ACCGCGC5101.8189894E-1210.14145931
ATCGGAA9100.09.6786332
GCGTGAG17500.09.63723122
ACAGGCG23900.09.59684818
TCGGAAG9050.09.4826853
CGTGAGC18200.09.39012323
Produced by FastQC (version 0.11.5)