FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10754873
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT150900.14030849085805105TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16450.025.9085431
ATCGGAA18650.023.3251192
CGTCTGA18250.023.2589416
ACACGTC18200.023.24042713
TCGGAAG18700.022.8614583
CGGAAGA20000.021.4504664
CACGTCT19900.021.33043514
ACGTCTG20150.020.99135215
GCACACG21050.020.59264411
CACACGT22700.019.02974512
AGAGCAC30100.014.60098
AGCACAC33550.013.45675410
GAGCACA33250.013.30750759
GAAGAGC34550.012.9811076
CAGTCAC34200.012.54407427
TCTGAAC35200.012.27202418
GTCACAG37150.011.8707229
GTCTGAA37100.011.72439517
CCAGTCA37650.011.55398226
CGTTGGA24550.011.4920331
Produced by FastQC (version 0.11.5)