FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7850790
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24400.013.6727491
AACGGGT5650.013.5398648
ACGGGAT15650.013.3706469
AAACGGG26600.013.3639357
GAAACGG27150.013.2591166
CGGGATT14050.012.81142610
GGAAACG27500.012.7631045
AACGGGA21650.012.5750948
ACGGGTT5050.011.5841939
CGCGCCC3907.8216544E-1110.96101333
CGCCCGG7700.010.81103135
ACAGGCG26250.010.62799518
GCGTGAG19750.010.48037622
GCGCCCG6050.010.04071234
CGTGAGC20650.09.69674823
ACCGCGC5701.8189894E-129.47322931
GATTTCG10750.09.4182135
CGGGTTT6000.09.37502910
CAGGCGT24550.09.34776119
CGGGTAT2701.0108626E-49.16669410
Produced by FastQC (version 0.11.5)