FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344733
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG439330.38725459647221316TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG49600.039.779345
CTCGTAT49900.039.58540744
TCTCGTA50450.039.06466343
ATCTCGT51500.038.0497742
AACGATC53000.037.1454738
ACGATCT55700.035.34488339
AAACGAT56150.035.22314537
GTCACGT57500.034.5109729
TGAAACG57600.034.45363635
CGTGAAA57950.034.4008633
GAAACGA58050.034.18655436
AGTCACG58150.034.08576628
GATCGGA59550.034.0080761
ACGTGAA59250.033.64488232
CACGTGA59500.033.5035231
TCACGTG60650.032.8297130
ATCGGAA62450.032.537522
ACACGTC62000.032.47688713
TCGGAAG63200.032.0445823
CGTCTGA63250.031.65720216
Produced by FastQC (version 0.11.5)