FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6432897
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4000.020.24669844
GATCGGA4200.019.857091
TATCTCG4050.019.44127541
GTCACGA4400.019.42864829
ATCGGAA5050.016.9451852
TCGTATG5400.016.6778245
TCTCGTA4850.016.23446543
ATCTCGT5000.015.74743142
CGGGATT12250.015.60969810
CGTCTGA5550.015.40289416
ACGGGAT12750.015.3504379
TCGGAAG5800.015.14226153
CGTTGGA19600.014.950321
CACGAGT5650.014.73211131
ACACGTC5950.014.36740613
AACGGGA17750.014.0681298
CGGAAGA6550.013.4084154
AAACGGG22200.013.2748627
ACGAGTG6350.013.10809932
GGAAACG21500.012.9878525
Produced by FastQC (version 0.11.5)