FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6808093
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8850.027.45681242
CGTATGC9050.026.6014244
TCGTATG9200.026.4122643
ACGGCTA9300.026.12844832
TCTCGTA9250.025.7828341
CGGCTAC9700.025.51489433
ATCTCGT9800.024.56541440
GATCGGA10500.023.5833721
GTCACGG10800.022.91581729
ACACGTC12000.021.37467813
CATCTCG11100.021.28299539
AGTCACG11750.021.2545328
CGTCTGA12450.020.602116
TCACGGC12150.020.18458630
ATCGGAA12450.020.0636232
ACGTCTG13700.018.88657815
CGGAAGA13350.018.711024
CACGGCT13300.018.60860631
TCGGAAG13450.018.5719053
CACGTCT14350.018.1878814
Produced by FastQC (version 0.11.5)