FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6032475
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16100.014.1800191
ACGGGAT10300.013.752749
CGGGATT9650.013.74708510
AAACGGG17100.013.281067
AACGGGA15300.012.9323418
GAAACGG18550.012.3650596
GGAAACG18900.012.1935885
GTTCGGT1400.00528249211.2437166
CCCGTAA3206.127884E-79.8891591
CGGGAAT7150.09.74858410
CCCCGAT2709.6132055E-59.2089521
ACAGGCG22600.09.05354918
ACCGCAC5556.366463E-118.91438131
CGTAGAA3309.649988E-68.9045251
CGGCTAA7350.08.8717139
GCCACCG14000.08.83339728
ACCGTGC5952.910383E-118.69305431
CCGCACC5504.947651E-108.58653832
CGTTGAG2400.00314333178.4764221
CCGTGCC6451.8189894E-118.36783332
Produced by FastQC (version 0.11.5)