FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8728907
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8650.029.65286642
TCTCGTA8850.028.47427441
CGTATGC9200.027.39054544
TCGTATG9600.026.71846843
GATCGGA10100.024.5184561
ATCTCGT10800.023.74974840
ACACGTC11300.023.4947913
ATCGGAA11300.022.7035562
TCGGAAG11700.022.6966193
CATCTCG11300.022.49963439
CGTCTGA12550.020.97527316
GTATGCC14350.018.65801845
CGGAAGA14650.018.4335354
ACGTCTG14750.017.69422115
CACGTCT15800.016.80323614
GCACACG16950.016.45953211
CACACGT18050.014.95786112
ATACGCC950.00538076314.21013234
AACGGGA23550.013.85288058
AAACGGG27300.013.59828957
Produced by FastQC (version 0.11.5)