FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4873437
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAA2755.984475E-912.2958171
ACGCCCG3551.7462298E-1011.40634334
ACCGCGC4900.011.018373531
CGCTCGA1450.006801320710.8682036
CGTTGGA19750.010.8431461
CGCCCGG6250.010.79800635
CACCGCG5350.010.51207730
CGTGAGC16300.010.21472423
AACGGGA16300.010.0748268
CGCGCCC4253.7471182E-1010.05696633
ACAGGCG21000.09.96244518
ACCACGC10750.09.83539331
AAACGGG20500.09.7678917
CCGCGCC5850.09.61360832
GCCACCG14300.09.59750828
CGGCTAA6700.09.40144239
GCGTGAG16300.09.38534722
ACGGGAT11750.09.3815369
CCGGCTA6500.09.3446238
GAAACGG21650.09.2546456
Produced by FastQC (version 0.11.5)