FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9423952
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5700.015.40690545
CTCGTAT6350.014.87982543
TCGTATG6350.013.81697944
TCTCGTA6800.013.56429342
TCCGTAT7200.013.43563536
CCGTATC7000.013.17674337
CGTCGAA1200.001659682413.12317824
CGTTGGA26350.012.9915241
TATCTCG7800.011.82528240
ACGGGAT16100.011.4584029
AAACGGG27500.011.1259647
ATCTCGT8400.010.98061941
AACGGGA22950.010.6848488
GAAACGG28450.010.6758236
AGTTCCG8300.010.29988533
GTTCCGT9250.010.21490734
ACCGCGC7150.010.0686431
CGGGATT15750.09.99893110
TTCCGTA9400.09.57324135
GGAAACG30600.09.4185055
Produced by FastQC (version 0.11.5)