FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5182172
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA750.001111015217.9998156
CGTCCGC1156.791005E-515.65186333
ACGTCCG1209.765927E-514.99941332
CGTTGGA14700.013.1873081
CCCGAAA2257.225208E-813.02374651
GTCCGCA1403.609396E-412.85750734
TCTCGTA2106.3096304E-611.78843743
AACGGGA12800.011.7751778
AAACGGG15150.011.73051557
TCCGCAC1802.3465125E-411.250319535
GAAACGG16100.011.1800096
ACGGGAT8900.011.121559
CGGGATT8600.010.98635110
CGGGAAT5500.010.63440810
GGAAACG17150.010.4968365
CCCGAAT4304.7093636E-99.4358091
AAATCGC3155.6639547E-69.2867835
CACCGCG3003.224218E-58.99955930
CCGCGCC2801.501963E-48.83893932
GCGTGAG11450.08.44855322
Produced by FastQC (version 0.11.5)