FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6168700
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA15900.013.9284531
ACGGGAT10900.013.4147179
AAACGGG18150.013.0141087
GAAACGG19100.012.7258216
AACGGGA15900.012.5917928
CGGGATT9700.012.5232410
GGAAACG19700.011.5385295
ACGTCCG1400.00526055511.25000432
CGCGCCC4051.546141E-1010.55461833
ACCGCGC5150.010.48544131
GCGTGAG14550.010.04951322
CGTGAGC14700.09.79393723
CACCGCG5301.8189894E-129.76415530
AACGGGT3951.2245437E-89.6815858
CGGGAAT6750.09.66471210
CCGCGCC5403.6379788E-129.58333732
CGCCCGG6350.09.210958535
ACAGGCG20700.09.12903118
CCCGAAT5453.7471182E-108.7075651
GCCACCG13500.08.6666728
Produced by FastQC (version 0.11.5)