FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845172
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT16850.012.3730559
CGTTGGA25650.012.0551731
CGGGATT15600.012.01835410
AACGGGA22350.011.8783968
AAACGGG27350.011.790737
GAAACGG27100.011.6227696
GGAAACG26350.011.558145
AACGGGT5350.010.9338458
CCCCGAT2157.191429E-79.7742441
CCCTAAA20950.09.4576561
CACCGCG4400.09.2040330
GCGTGAG13700.09.19651322
CAAAGCG13550.09.1945911
CGTGAGC14450.09.03058523
CCCAAAG70850.08.8982181
GCTAGAC15450.08.83445729
GAGCCAC41100.08.83162226
CCCGAGT12450.08.8013061
ACAGGCG18250.08.79402918
ACGGGTT4450.08.7634559
Produced by FastQC (version 0.11.5)