FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12873330
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9700.028.06576745
GATCGGA13000.027.8927481
CTCGTAT16950.020.9725244
TCTCGTA17250.020.7382143
TATCTCG17900.019.8592241
GTCACGA18300.019.30219729
TCGTATG18800.019.14808745
AGTCACG20550.017.73623328
ATCGGAA21250.017.593732
ATCTCGT20800.017.52326242
ACGAGTG21150.017.23307832
TCGGAAG21850.017.197573
ACACGTC21550.017.12267713
CGTCTGA21700.016.69267716
CACGAGT22000.016.56725531
ACGTCTG23200.015.80742715
CGGAAGA24700.015.4853844
CACGTCT24900.014.63790514
GCACACG28000.013.25870111
TCACGAG30950.012.79411330
Produced by FastQC (version 0.11.5)