FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034403
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG15200.010.26237222
ACAGGCG20700.010.14415118
CGTTGGA18600.010.1025711
CCCAAAG64800.09.998551
ACGGGAT13050.09.88439
CCCAAGT29300.09.850731
CGTGAGC16150.09.84450923
CACCGCG7550.09.73441530
CGGGATT12750.09.52868310
AACGGGA17600.09.3742838
AAACGGG21500.09.3481227
CCCCGGA3254.4019544E-109.2508461
GAGCCAC42050.09.23835226
GCCACCG15500.09.19290228
CCCGAGT12450.09.1765411
GAAACGG20500.08.9261466
GGAAACG19850.08.7650725
CACTGCG5850.08.71733630
CCCTAAT11350.08.6089921
TACAGGC49600.08.58805217
Produced by FastQC (version 0.11.5)