FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7635021
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21800.013.2421341
CGGGATT13700.011.82333610
AAACGGG24350.011.64125257
AACGGGA19950.011.6150098
CCCGAAA3501.3096724E-1011.5986921
GAAACGG25050.011.5001596
ACGGGAT14750.011.4392449
GGAAACG25900.010.4272965
AACGGGT5650.09.954448
CACCGCG5658.3127816E-108.36227730
CGGGAAT9850.08.2223210
GATCGGA2550.00554007947.95988661
CGCCCGG7355.456968E-127.95839535
CGCGCCC4551.0864132E-67.91125233
ACCGCGC6053.1632226E-97.80939931
CCGCGCC6103.7125574E-97.745387632
AAGCGTA2700.0094487137.4990118
GATTTCG8451.8189894E-127.4574715
GCGTGAG18000.07.374655222
CGTGCAT3400.00117902677.2965621
Produced by FastQC (version 0.11.5)