FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7527443
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA18500.014.8356118
CGTTGGA21750.014.65214351
AAACGGG22100.014.5566397
ACGGGAT14300.014.1587129
GAAACGG23450.013.6226886
CGGGATT13600.013.5646810
GGAAACG24500.012.8552125
ACCGCGC6300.011.4284531
CCACGAT4353.6379788E-1210.9111721
CGCGCCC4651.8189894E-1210.64455233
CACCGCG6950.010.35960230
CGATAGT2453.3190463E-510.1477081
CCGCGCC6900.010.10858732
CACGATT3603.014975E-89.9986972
CCCGGGT11450.09.8697441
CGGGAAT8300.09.7579610
GCGTGAG19150.09.63333922
CGCGCCT3053.7663413E-69.58961633
GCCACCG16900.09.58569728
AACGGGT4752.8012437E-109.4722628
Produced by FastQC (version 0.11.5)