FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9757793
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7750.016.2652551
AGTCACG7400.016.21598828
ACACGTC7100.016.05561813
GTCACGC7800.015.76901129
CGTCTGA7650.015.29343316
ATCGGAA8750.014.0578822
ACGTCTG8550.013.85902815
CACGTCT8800.013.63575414
TCGGAAG9300.013.2260963
CACACGT10250.011.41412412
GCACACG10850.011.33589811
CGGAAGA10750.011.3025154
CGTTGGA23950.010.58923151
GCGTGAG16250.010.43078322
CCCAAAG73900.010.2548711
GAGCCAC45600.010.23022626
CGTGAGC17050.010.20529323
CCCAAGT31150.010.0686571
ACGGGAT15500.09.8705269
CCCTAAA22450.09.7593251
Produced by FastQC (version 0.11.5)